du145 human prostate cancer cells  (ATCC)

Journal: ACS Applied Materials & Interfaces

Article Title: Site-Specific Abiraterone Protein–Drug Conjugates via Hedgehog Autoprocessing

doi: 10.1021/acsami.5c22490

Figure Lengend Snippet: Schematic of hedgehog-mediated abirateronylation as a noncanonical PTM (ncPTM) for generating protein–drug conjugates (PDCs). An intrinsically disordered protein polymer (IDPP) serves as the model protein scaffold. The resulting hybrid biopolymers are internalized by DU145 cells and exert cytotoxicity. Created with BioRender.com

Article Snippet: DU145 human prostate cancer cells (ATCC HTB-81) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Polymer

Journal: ACS Applied Materials & Interfaces

Article Title: Site-Specific Abiraterone Protein–Drug Conjugates via Hedgehog Autoprocessing

doi: 10.1021/acsami.5c22490

Figure Lengend Snippet: Biophysical and biological assessment of the optimized PDC and controls. (a) Hydrodynamic radius ( R h ) of (A,40) carrier (E), E–Abi and E–Chol at 50 μM and 200 μM, measured by dynamic light scattering. E–Abi forms reversible, concentration-dependent oligomers. (b) Release kinetics of conjugated sterols (E-Abi, E–Andro and E–Chol) in PBS (pH 6.5) at 37 °C, along with the chemical structures of Abi and Andro tail groups. E–Abi and E–Andro shows sustained release, whereas E–Chol displays negligible sterol release over the same period. Shaded areas represent the 95% confidence intervals of the nonlinear regression fit to a first-order kinetics model. (c) Dose–response curves for DU145 prostate cancer cells after 72 h exposure to free drug, conjugate, and controls (measured by MTT assay; nonlinear dose–response fit). (d) Viability of 3D DU145 spheroids after 24-h treatment with 100 μM of each compound, showing superior efficiency of E–Abi. (e) Representative live/dead fluorescence images of treated spheroids. Live cells are stained green (calcein AM), and dead cells are stained red (propidium iodide). Data are mean ± s.d. ( n = 3). Statistical analysis was performed using one-way ANOVA with posthoc tests (Tukey’s for a, Holm–Sidak’s for d): * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Article Snippet: DU145 human prostate cancer cells (ATCC HTB-81) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Concentration Assay, MTT Assay, Fluorescence, Staining

Journal: ACS Applied Materials & Interfaces

Article Title: Site-Specific Abiraterone Protein–Drug Conjugates via Hedgehog Autoprocessing

doi: 10.1021/acsami.5c22490

Figure Lengend Snippet: Lipidation-dependent modulation of biopolymer penetration in 3D tumor spheroids. (a–c) Representative bivariate flow cytometry dot plots of WGA-Alexa Fluor 680 (membrane label) versus carrier-Alexa Fluor 488. The gated population represents WGA + /Carrier + cells, and the percentage of uptake-positive cells is indicated for each condition. DU145 spheroids were incubated overnight with labeled (a) E, (b) E–Abi, (c) E–Chol, dissociated to single cells, and analyzed by flow cytometry. (d) Bar graph summarizing the fraction of uptake-positive cells across treatments. (e–g) Confocal z-stack images of DU145 spheroids treated with E, E–Abi, or E–Chol for 24 h. WGA (AF680, red) marks the cell membrane, and constructs (AF488, green) indicate carrier localization. Data are mean ± s.d. ( n = 3). Statistical significance was determined using one-way ANOVA with Tukey’s multiple comparisons test (** p < 0.01).

Article Snippet: DU145 human prostate cancer cells (ATCC HTB-81) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 0.1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Flow Cytometry, Membrane, Incubation, Labeling, Construct

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Concentration Assay

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Membrane, Fluorescence, Control

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Control

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Staining

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Staining

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA promotes the migration and invasion of PC3 and DU145 cells. ( A ) PC3 and DU145 cells were seeded in the upper chamber of Transwell devices and treated with BPA for 24 h, followed by crystal violet staining of migrated cells. ( B ) Cells were seeded in the upper chamber of Transwell devices coated with Matrigel and allowed to invade for 24 h, after which, the cells were stained and counted. Results are expressed as the means ± SD of three replicate experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Migration, Staining

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA regulates protein levels of EMT biomarkers and transcription factors in prostate cancer cells. ( A , B ) After 24 h of BPA treatment, the expression levels of N-cadherin, vimentin, MMP2, MMP9, and MT1-MMP were measured in PC3 and DU145 cells. ( C , D ) After 24 h of BPA treatment, the expression levels of Snail, Slug, and Twist1 were measured in PC3 and DU145 cells. Data are shown as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Expressing, Standard Deviation

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA activates the PI3K/AKT/GSK-3β/β-catenin signaling pathway.( A , B ) BPA enhanced the PI3K/AKT/GSK-3β/β-catenin signaling pathway in a dose-dependent manner; cells were treated with BPA at different concentrations for 24 h. ( C , D ) BPA reversed the effect of the PI3K inhibitor LY294002 on the PI3K/AKT/GSK-3β/β-catenin signaling pathway in prostate cancer cells. Data are shown as the mean ± standard deviation of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Standard Deviation

Journal: Oncology Research

Article Title: Elucidating the Potential Targets and Mechanisms of Bisphenol A-Induced Prostate Cancer Based on Network Toxicology and Molecular Docking Analyses

doi: 10.32604/or.2026.076716

Figure Lengend Snippet: BPA promotes the migration and invasion of PC3 and DU145 cells. ( A ) PC3 and DU145 cells were seeded in the upper chamber of Transwell devices and treated with BPA for 24 h, followed by crystal violet staining of migrated cells. ( B ) Cells were seeded in the upper chamber of Transwell devices coated with Matrigel and allowed to invade for 24 h, after which, the cells were stained and counted. Results are expressed as the means ± SD of three replicate experiments. *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell lines were obtained from Shanghai Model Organisms Company (Shanghai, China; catalog no. NM-H003 and NM-H004).

Techniques: Migration, Staining